Journal: Sensors (Basel, Switzerland)

Article Title: 3D-Printed Bubble-Free Perfusion Cartridge System for Live-Cell Imaging

doi: 10.3390/s20205779

Figure Lengend Snippet: ( a ) Typical dose–response profiles of fluorescence intensity changes in Or13a cells in the 3D-printed cartridge using a 4× objective lens during the addition of 1-octen-3-ol at concentrations ranging from 30 nM to 3 µM. The long grey bar indicates the perfusion of the assay buffer solution including 0.1% DMSO by peristaltic pumps. ( b ) Bright field and baseline fluorescence images of Or13a cells observed by a 4× objective lens. ( c ) Each point of fluorescence intensity change in ( b ) observed by a 4× objective lens. The size of each observation field is 400 × 400 µm 2 . ( d ) Difference between response times of Area 1 and Area 2, and differences between response times of Area 3 and Area 4 stimulated by 3 µM 1-octen-3-ol. Data represent the mean ± SEM of calculated time differences between response time of each 400 × 400 µm 2 area ( N = 4 individual tests). ( e ) CFD simulation result of flow velocities in the 3D-printed cartridge. These velocities of the cross-sectional view of B–B direction in . ( e ) is represented as a contour. Scale bars: ( b ) 500 µm; ( e ) 1 mm.

Article Snippet: Flow dynamics in the designed cartridge were simulated using commercial computational fluid dynamics (CFD) software (ANSYS 18; ANSYS, Inc., Canonsburg, PA, USA).

Techniques: Fluorescence